ABSTRACT
The freeze and thaw (F&T) step in bioprocessing is critical to product integrity, sterility, and scalability. In this webinar, we’ll guide you through selecting the right consumables and hardware for your F&T process, considering molecule characteristics, batch volume, and facility constraints. We’ll compare bottles vs. single-use bags and demonstrate why bags are the preferred option in modern cryopreservation strategies. Learn how Celsius solutions improve handling and sterility, and explore how to quantify benefits using our Total Cost of Ownership (TCO) tool. Real-world case studies and customer experiences will highlight practical advantages. Whether you're maintaining legacy processes or preparing for a transition, this session will equip you with the knowledge to make informed, confident decisions.
2:00 - 2:15
How to Select What to Use in F&T Process
- What is the best way to decide what kind of products from F&T to choose from. Eg molecule, process.
- When to select different consumables and hardware – based on molecule robustness, volume per batch.
- Overview of F&T process and criteria to know what to select.
- Cryopreservation process.
2:15 - 2:20
Share Sases of Customers Using Bottles for F&T
- Share why these are still existing in the market.
- Legacy molecule still uses bottles and we cannot change this.
- Compare bottle and bag filling / freezing - Benefits of bags over bottles.
2:20 - 2:25
What to Consider if You Want to Make Changes to Your Process
- Existing infrastructure, change in MOC of consumable, Validation, Studies etc.
- Importance of having similar technologies across DS and DP sites.
- What must end user consider if they are switching from bottles to bags, comparing pros and cons of bottles to bags.
- End to end solution available for bags.
2:25 - 2:35
Why was Celsius Bags Designed This Way?
- Freezing dynamics in bottle vs bag.
- Design of the bag, rationale of selecting and designing the bag this way and also in selected sizes – aim is to ensure sterility and aseptic during operation, while making it ergonomic during transportation and operational handling. (Bottles – not ergonomic and user friendly in handling, limited in size, not 100% aseptic as there is a need to open the cap during BDS filling.
2:35 - 2:45
TCO – How This TCO Tool was Designed?
- To run through TCO using examples prepared. End user can reach out to us if they have more enquiries.
- Show where is the saving when converting bottles to bags.
2:45 - 2:50
Customer to Share Experience with Celsius?
- To check if customer is willing to provide testimony. They can talk about ease of use / time reduction/ long term cost etc.
2:50 - 3:00
Live Questions and Answers
Stefan Woodhouse
Product Manager Freeze and Thaw
Sartorius
Stefan Woodhouse is the Product Manager – Freeze & Thaw at Sartorius. He holds a Master degree and a PhD in Biochemical Engineering where his research was focused on improving product titres in microbial fermentation. Stefan joined Sartorius in 2016 and has worked in various technical field roles, most recently as product specialist for Freeze & Thaw EMEA, providing application and project support to customers in the region. Since 2024 as product manager, he’s been dedicated to providing robust solutions for the freezing and transport of valuable drug substances to the market. Most recently this was achieved by the expansion of the Celsius® FFT | FFTp portfolio with the FFTp 2L, and completing the launch of the second generation of products.
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